Anti-HIV ribozymes

Details Anti-HIV ribozymes Anti-HIV ribozymes

1997-08-12

1999-11-16

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

435 6, 435 9131, 4353201, 435325, 536 231, 536 232, 536 245, C12N 1585, C07H 2104, C12Q 168

Patent

active

059856610

ABSTRACT:
Ribozymes targeted against two portions of the HIV-1 genome were designed to cleave HIV RNA in the tat gene (TAT) or in a common exon for tat and rev (TR). The ribozymes were cloned into the LN (LTR-neomycin) retroviral vector plasmids and expressed as part of viral LTR-driven transcripts. The vectors were packaged as amphitropic virions and used to transduce human T-lymphocytes. Expression of the vector transcripts containing the ribozyme sequences were readily detected by Northern blot analysis of the transduced T cells. The T-lymphocytes expressing the anti-HIV-1 ribozymes showed resistance to HIV-1 replication.

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