Anti-gp130 monoclonal antibodies

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Monoclonal antibody or fragment thereof

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4241531, 4241731, 435 7021, 4351722, 43524027, 5303871, 53038822, 5303887, 53038885, 5303913, 5303896, A61K 39395, C07K 1628, C12P 2108, C12N 520

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055715133

ABSTRACT:
Anti-gp130 monoclonal antibodies (Mabs) obtained from hybridomas designated 4B11 and 2H4 are effective in the inhibition of the acute phase response on hepatoma cells and prevent the IL-6-induced growth inhibition of A375 cells in vitro. Administration of the antibodies to dogs showed that 2H4 is a potent in vivo inhibitor of the IL-6-induced acute phase response, abrogating IL-6-mediated-increments in fibrinogen, C-reactive protein and the platelet count. Antibodies may be used in methods for measuring soluble gp130 and in therapeutic treatments. The 2H4 antibody may be used in inhibiting in vivo the function of gp130 or cellular factors dependent on gp130 for cellular transduction.

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Mackiewicz et al., "Complex of Soluble Human IL-6-Receptor/IL-6 Up-Regulates Expression of Acute-Phase Proteins", The Journal of Immunology, vol. 149, 2021-2027, Sep. 15, 1992.
Taga et al., "Functional Inhibition of Hematopoietic and Neurotrophic Cytokines by Blocking the Interleukin 6 Signal Transducer gp130", Proc. Natl. Acad. Sci. USA, vol. 89, 10998-11001, Nov. 1992.
Narazaki et al., "Soluble Forms of the Interleukin-6 Signal-Transducing Receptor Component gp130 in Human Serum Possessing a Potential to Inhibit Signals Through Membrane-Anchored gp130", Blood, vol. 82, No. 4, 1120-1126, Aug. 15, 1992.
Wendling et al., "Treatment of Severe Rheumatoid Arthritis By Anti-Interleukin 6 Monoclonal Antibody", The Journal of Rheumatology, 20:259-262, 1993.
Saito et al., "Preparation of Monoclonal Antibodies Against the IL-6 Signal Transducer, gp130, That Can Inhibit IL-6-mediated Functions", Journal of Immunological Methods, 163:217-223, 1993.

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