Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-10-22
1998-02-03
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 721, 436519, 530300, G01N 3353, G01N 33554, C07K 400
Patent
active
057143323
ABSTRACT:
Compounds and libraries are labeled with a galactosyl epitope amd then screened in accordance with an assay involving cells having a characteristic of interest. Conveniently, the screening may embody target cells, where the compounds are brought in contact with the cells. Each of the compounds carries with it the information of its identity or method of synthesis. After washing away non-specifically bound compounds, blood may be applied to the cells, whereby antibody binding to the galactosyl epitope initiates the complement cascade. Plaques are identified and the compound associated with the plaque identified. The formation of the plaque demonstates that the compound has specific affinity for the target cell, binding of the compound to the cell does not interfere with binding of the antibody, and that the complex is capable of cytotoxic activity by means of the complement cascade.
REFERENCES:
Guerif, F., et al., "Screening of a Pig cDNA Expression Library with Human Serum in Order to Identify New Xenoantigens," Cambridge Sci. Abs., Acc. Nr.: 01971387 3820007, 1995., Transplantation proceedings, 27(4):2941 (1995).
Galili, U., et al., "Evolutionary Relationship Between the Natural Anti-Gal Antibody and the Gal.alpha.1.fwdarw.3Gal Epitope in Primates," PNAS, USA, 84:1369-1373 (1987).
Galili, U., et al., "One Percent of Human Circulating B Lymphocytes are Capable of Producing the Natural Anti-Gal Antibody," Blood, 82(8):2485-2493 (1993).
Galili, U., et al., "Human Natural Anti-.alpha.-Galactosyl IgG. II. The Specific Recognition of .alpha.(1.fwdarw.3)-Linked Galactose Residues," J. Exp. Med., 162:573-582 (1985).
Buelow Roland
Lussow Alexander R.
Pouletty Philippe
SangStat Medical Corporation
Scheiner Toni R.
Trecartin Richard F.
Ungar Susan
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