Anti-acharan sulfate antibody and its application

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S388200, C435S007100, C435S070210, C435S810000

Reexamination Certificate

active

07915389

ABSTRACT:
An anti-acharan sulfate antibody, a hybridoma that produces the antibody, a detection method and a detection kit to which the antibody is applied are disclosed. The anti-acharan sulfate antibody can be produced by immunizing a mammal using as an antigen a substance obtained by chemically bonding a protein to acharan sulfate.

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Dam et al, J Biol Chemistry 279(37): 38346-38352, Sep. 2004.
Harlow et al, in Antibodies a Laboratory Manual, 1988, Cold Spring harbor laboratory publication, Cold Spring Harbor, NY, chapter 5, pp. 56-59, 72-81.
Harlow et al, in Antibodies a Laboratory Manual, 1988, Cold Spring harbor laboratory publication, Cold Spring Harbor, NY, chapter 6, pp. 139-154.
Joo, et al. “Nucleolin: Acharan Sulfate-binding Protein on the Surface of Cancer Cells,”Glycobiology, vol. 15, No. 1, pp. 1-9, Jan. 2005.
Kim, et al. “A New Glycosaminoglycan from the Giant African SnailAchatina fluica,” The Journal of Biological Chemistry, vol. 271, No. 20, pp. 11750-11755, May 17, 1996.
ten Dam, et al. “Detection of 2-O-Sulfated Iduronate andN-Acetylglucosamine Units in Heparan Sulfate by an Antibody Selected Against Acharan Sulfate (IdoA2S-GlcNAc)n”The Journal of Biological Chemistry, vol. 279, No. 37, pp. 38346-38352, Sep. 10, 2004.

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