Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-02-12
1997-01-07
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4353201, 536 234, 536 237, 530350, 530402, C07K 1900, C12N 1531, C12N 1570
Patent
active
055916317
ABSTRACT:
The present invention provides a nucleic acid encoding a fusion protein, comprising a nucleotide sequence encoding the protective antigen (PA) binding domain of the native lethal factor (LF) protein and a nucleotide sequence encoding an activity inducing domain of a second protein. Also provided is a nucleic acid encoding a fusion protein, comprising a nucleotide sequence encoding the translocation domain and LF binding domain of the native PA protein and a nucleotide sequence encoding a ligand domain which specifically binds a cellular target. Proteins encoded by the nucleic acid of the invention, vectors comprising the nucleic acids and hosts capable of expressing the protein encoded by the nucleic acids are also provided. A composition comprising the PA binding domain of the native LF protein chemically attached to a non-LF activity inducing moiety is further provided. A method for delivering an activity to a cell is provided. The steps of the method include administering to the cell a protein comprising the translocation domain and the LF binding domain of the native PA protein and a ligand domain, and administering to the cell a product comprising the PA binding domain of the native LF protein and a non-LF activity inducing moiety, whereby the product administered is internalized into the cell and performs the activity within the cell.
REFERENCES:
Klimpel, Kurt R., et al. (1992) "Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin", Proceedings of the National Academy of Sciences of USA, 89(21):10277-10281.
Arora, Navene, et al. (1993) "Residues 1-254 of anthrax toxin lethal factor are sufficient to cause cellular uptake of fused polypeptides", Journal of Biological Chemistry, 268(5): 3334-3341.
Robertson et al "Molecular cloning and expression in E. coli of the lethal factor gene of B. anthracis", Gene 44:71-78 (1986).
Bartkus et al "Transcriptional Regulation of the Protective Antigen Gene of Bacillus anthracis", Inf. Immun. 57(8):2295-2300 (Aug. 1989).
Iacono-Connors et al, "Protection against Anthrax with Recombinant Virus Expressed Protective Antigen . . . ", Inf. Immun. 59(6):1961-1965 (Jun. 1991).
Cataldi et al, "Regulation of pag gene expression in Bacillus anthracis . . . " FEMS Microbiol. Lett. 98:89-94 (Nov. 1992).
Walz et al, "Sequential effects of ILZ-diphtheria toxin fusion protein on T-cell activation", PNAS 86: 9485-9488 (Dec. 1989).
Arora et al. J. Bio. Chem. 267(22):15542-15548, 1992.
Arora and Leppla Abstract:ASM Annual Meeting, New Orleans, LA, May 1992, p. 31, #B-33.
Arora et al. Abstract:Third Internatl. Sympos. on Immunotoxins, Orlando, FL, Jun. 19-21, 1992.
Quinn et al. J. Bio. Chem. 266(30):20124-20130, 1991.
Oeltmann and Frankel News 5:2334-2337, Jul. 1991.
Singh et al. J. Bio. Chem. 266(23):15493-15497, 1991.
Singh et al. J. Bio. Chem. 264(32):19103-19107, 1989.
Leppla et al. Abstract:Fifth European Workshop on Bacterial Protein Toxins Veldhoven, Jun. 30-Jul. 5, 1991.
Klimpel et al. Abstract:1992 ASM General Meeting, New Orleans, LA, May 1992, p. 31, #B-32.
Arora Naveen
Klimpel Kurt R.
Leppla Stephen H.
Nicholls Peter J.
Singh Yogendra
The United States of America as represented by the Department of
Walsh Stephen G.
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