Anthrax lethal factor is a mapk kinase protease

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S004000, C435S194000

Reexamination Certificate

active

07056693

ABSTRACT:
The present invention relates to in vitro and ex vivo methods of screening for modulators, homologues, and mimetics of lethal factor mitogen activated protein kinase kinase (MAPKK) protease activity, as well as methods of treating cancer by administering LF to transformed cells.

REFERENCES:
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Kimpel, K.R., et al., “Anthrax toxin lethal factor contains a zinc metalloprotease consensus sequence which is required for letal toxin activity,”Molecular Microbiology, 1994, pp. 1093-1100, vol. 13, No. 6.
Koo, Han-Mo, et al., “Enhanced Sensitivity to 1-β-D-Arabinofuranosylcytosine and Topoisomerase II Inhibitors in Tumor Cell Lines Harboring Activited ras Oncogenes,”Cancer Research, Nov. 15, 1996, pp. 5211-5216, vol. 56.
Menard, Armelle, et al., “The cytotoxic activity of Bacillus anthracis lethal factor is inhibited by leukotriene A4hydrolase and metallopeptidase inhibitors,”Biochem. J., 1996, pp. 687-691, vol. 320.
Oka, H., et al., “Constitutive Activation of Mitogen-Activated Protein (MAP) Kinases in Human Renal Cell Carcinoma,”Cancer Res., 1995, pp. 4182-4187, vol. 18.
Weinstein, John N., et al., “An Information-Intensive Approach to the Molecular Pharmacology of Cancer,”Science, Jan. 17, 1997, pp. 343-349, vol. 275.

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