Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2006-11-07
2006-11-07
Mehta, Ashwin D. (Department: 1638)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100, C435S419000, C536S024100
Reexamination Certificate
active
07132292
ABSTRACT:
The present invention provides novel anther-specific genes, their promoters, and uses of the same. Rice seedlings were treated with gibberellin, and then genes and proteins with up-regulated expression were screened using DNA microarray analysis and proteome analysis. As a result, eight types of β-tubulins were identified. Among them, OsTUB8 was expressed specifically in the anther. Thus OsTUB8 was suggested to be involved in male sterility in rice, and it was thought that regulating OsTUB8 expression in plants could alter plant fertility. In addition, OsTUB8 promoters were thought to comprise anther-specific activity. Thus, the present invention can be said to be highly valuable when used as a tool for anther-specific gene expression.
REFERENCES:
Kim et al. (1994) A 20 Nucleotide Upstream Element is Essential for the Nopaline Synthase (nos) Promoter Activity. PMB vol. 24, pp. 105-117.
Buell R. (2003) GenBank Accession #AC139174.
Yashikawa et al. (2003) Expression Analyses of B-tubulin Isotype Genes in Rice. Plant Cell Physiol. vol. 44, pp. 1202-1207.
Jeon et al. (1999) Isolation and Characterization of an Anther-Specific Gene, RA8, from Rice (Oryza sativa L.). PMB vol. 39, pp. 35-44.
Yoshikawa, M., et al., “Expression Analyses of β-tubulin Isotype Genes in Rice,”Plant Cell Physiol. 44:1202-1207, Oxford University Press (Nov. 2003).
Komatsu Setsuko
Yang Guangxiao
Yoshikawa Manabu
Kingdon Worley Cathy
Mehta Ashwin D.
National Institute of Agrobiological Sciences
Sterne Kessler Goldstein & Fox P.L.L.C.
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