Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal in an in vivo...
Reexamination Certificate
2005-05-24
2005-05-24
Leffers, Gerry (Department: 1636)
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal in an in vivo...
C424S009200, C424S009800
Reexamination Certificate
active
06897352
ABSTRACT:
Non-human mammalian animals having a higher epidermal expression level of protein kinase Cε than their wild-type counterparts are phenotypically distinguished from wild-type animals in that the animals induced to develop tumors in a chemical initiation/promotion protocol are suppressed for subsequent papilloma development but are susceptible to developing squamous cell carcinoma and metastatic squamous cell carcinoma. The animals are advantageously used in methods for screening putative agents for altering the susceptibility, development and progression of squamous cell carcinoma and metastatic squamous cell carcinoma and have further commercial value as tools for investigating the development of metastatic disease.
REFERENCES:
patent: 6521815 (2003-02-01), Verma et al.
Reddig et al. Transgenic mice overexpressing protein kinase C epsilon in their epidermis exhibit reduced papilloma burden but enhance carcinoma formation after tumor promotion. Cancer Research, vol. 60, pp. 595-602, 2000.*
Sigmund. Viewpoint: Are studies in genetically altered mice out of control? Arteriosclerosis, thrombosis, and vascular biology. vol. 20, No. 6, pp. 1425-1429, 2000.*
Wall. Transgenic livestock: Progress and prospects for the future. Theriogenology. vol. 45, No. 1, pp. 57-68, 1996.*
Nair et al. UVB-induced pigmentation in hairless mice as an in vivo assay for topical skin-depigmenting activity. Skin Pharmacology. vol. 2, No. 4, pp. 187-197, 1989.*
Tseng, C.-P. and Verma, A.K. (1996). Gene 169:287-288.
Fujii, M. (1997). J. Stomatol. Soc. 74(1):52-66.
Baxter, G., et al., “Constitutive presence of a catalytic fragment of protein kinase CE in a small cell lung carcinoma cell line,” J. Biol. Chem. 267:1910-1917 (1992).
Cacae, A.M., et al., “The E isoform of protein kinase C is an oncogene when overexpressed in rat fibroblasts,” Oncogene 8:2095-2104 (1993).
Denning, M.F., et al., “Specific protein kinase C isozymes mediate the induction of keratinocyte differentation markers by calcium,” Cell Growth Differ. 6:149-157 (1995).
DiGiovanni, J., “Multistage carcinogenesis in mouse skin,” Pharmacol. Ther. 54:63-138 (1992).
Hennings, H., et al., “FVB/N mice: an inbred strain sensitive to the chemical induction of squamous cell carcinomas in the skin,” Carcinogenesis 14:2353-2358 (1993).
Mills, K.J., et al., “Alterations in protein kinase C isozymes a and B II in activated Ha-ras-containing papillomas in the absence of an increase in diacylglycerol,” Carcinogenesis 13:1113-1120 (1992).
Mischak, H., et al., “Overexpression of protein kinase C-8 and -E in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenecity,” J. Biol. Chem. 268:6090-6096 (1993).
Ono, Y., et al., “The structure, expression, and properties of additional members of the protein kinase C family,” J. Biol. Chem. 263:6927-6932 (1988).
Perletti, G.P., et al., “Overexpression of protein kinase CE is oncogenic in rat colonic epithelial cells,” Oncogene 12:847-854 (1996).
Reddig, P.J., et al., “Transgenic mice overexpression protein kinase C8 in the epidermis are resistant to skin tumor production by 12-O-Tetradecahoylphorbol-13-acetate,” Cancer Research 59:5710-5718 (1999).
Taketo, M., et al., “FVB/N: An Inbred Mouse Strain Preferable for Transgenic Analyses” PNAS USA 88:2065-2069 (1991).
Verma, A.K., “Protein kinase C and skin cancer prevention,” In: H. Mukhtar ed. Skin Cancer: Mechanisms and Human Relevance, Ed. 1:383-390 (1995).
Wang, X.J., et al., “Loss of mouse epidermal protein kinase C isozyme activities following treatment with phorbol ester and non-phorbol ester tumor promoters,” Carcinogenesis 15:2795-2803 (1994).
Jansen, A.P., et al., “Relation of the Induction of Epidermal Ornithine Decarboxylase and Hyperplasia to the different skin tumor-promotion susceptibilities of protein kinase Ca, -8, and -E transgenic mice,” Int. J. Cancer 93:635-643 (2001).
Verma Ajit K.
Wheeler Deric L.
Dunston Jennifer
Leffers Gerry
Quarles & Brady LLP
Wisconsin Alumni Research Foundation
LandOfFree
Animal model system for photodamage, photoaging and skin... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Animal model system for photodamage, photoaging and skin..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Animal model system for photodamage, photoaging and skin... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3426684