Animal genotyping method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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07629119

ABSTRACT:
This invention relates to a method for determining whether a bovine animal possesses a gene for β-casein A2or the gene for β-casein A1in its milk by testing the DNA of the bovine animal for the presence of at least one DNA marker (excluding those DNA markers present in the sequence of the genes themselves). In particular, the method uses SNPs as DNA markers for the β-casein A2and β-casein A1genes.

REFERENCES:
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patent: 6570060 (2003-05-01), McLachlan
patent: 96/14577 (1996-05-01), None
patent: WO-96-36239 (1996-11-01), None
Martin et al., “The impact of genetic polymorphisms on the protein composition of ruminant milks”, Reprod. Nutr. Dev. 42, 2002, pp. 433-459.
Groves et al., “Composition of Bovine γ-Caseins A1and A3, and Further Evidence For a Relationship in Biosynthesis of γ- and β-Caseins”, J Dairy Sci, vol. 55, No. 8, 1972, pp. 1041-1049.
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AS Truswell, “Review—The A2 milk case: a critical review”, European Journal of Clinical Nutrition (2005), 59, pp. 623-631.
Lien S et al.: “A Simple and Powerful Method for Linkage Analysis by Amplification of DNA from Single Sperm Cells” Genomics, Academic Press, San Diego, US vol. 16, No. 1, dated Apr. 1, 1993 pp. 41-44, XP024797158.
Groenen M A M et al. “The complete sequence of the gene encoding bovine alpha2-casein” Gene, Elsevier, Amsterdam, NL vol. 123, No. 2, Jan. 30, 1993, p. 187-193, XP023183961.
Martin p et al.: “The impact of genetic polymorphisms on the protein composition of ruminant milks” Sep. 1, 2002, Reproduction, Nutrition, Development, Paris, FR, pp. 433-459, XP008088224.
European Patent Office issued a Supplementary European Search Report dated Jan. 16, 2009, Application No. 03 72 8181.

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