Animal cell transformant, method for obtaining the transformant

Details Animal cell transformant, method for obtaining the transformant Animal cell transformant, method for obtaining the transformant

1987-05-04

1993-06-15

Ellis, Joan

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 91, 4351723, 4352402, 4353201, 4352351, 536 232, 530350, 935 9, 935 32, 935 34, 935 42, 935 57, 935 61, 935 70, C12P 2102, C12N 1500, C12N 500, C12N 700

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052197387

ABSTRACT:
By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 .mu.M or lower, both genes can be amplified effectively within an MTX resistant strain and a stable amplified transformant can also be obtained. The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant.

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