Animal cell culture

Details Animal cell culture Animal cell culture

1995-02-28

1997-09-30

Feisee, Lila

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524026, 43524027, 4352403, 43524031, C12N 502, C12N 506, C12N 510, C12N 520

Patent

active

056725027

ABSTRACT:
Process for the fed-batch culture of animal cells comprises culturing the cells in nutrient medium characterized in that during the culture the medium is supplemented with a combined feed of one or more energy sources and one or more animo acids, and culturing is continued into the decline phase of the culture to provide the product(s) of the cells. The process is particularly applicable to genetically modified cells, especially hybridoma cell cultures to produce monoclonal antibodies. Preferably, the supplemental feed is fed to the culture at a slow rate over a prolonged period. Very significant enhancement of overall product yield may be obtained.

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