Angiogenic factor production

Details Angiogenic factor production Angiogenic factor production

1983-11-29

1987-10-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

424 95, 435240, 435241, 536 21, 514 56, A61K 3512, C12P 100, C08B 3710

Patent

active

046983015

ABSTRACT:
New angiogenic factors of low molecular weight (under 1000), useful as treatment agents for wound healing and for biological assaying, made from in vitro cultures of a wide variety of tumor cells by a treatment which breaks up the proteinaceous tumor angiogenesis factors ("TAF") in them and liberates the more mobile low molecular weight factors. The treatment can be applied to cell extracts or the aqueous portion of the culture, and the proteinaceous part may be rendered insoluble or captured chemically so as to release the new factor. Separation techniques which can be used include solvent extraction of dried liquid fractions, using polar solvents such as ethanol, and the liberated factor can be purified chromatographically using adsorbents for which the factor has affinity, especially a basic adsorbent and preferably polysaccharide-based, followed by elution.

REFERENCES:
patent: 4268629 (1981-05-01), Tolbert et al.
patent: 4273871 (1981-06-01), Tolbert et al.
patent: 4335215 (1982-06-01), Tolbert et al.
Weiss et al., Br. J. Cancer 1979, vol. 40, pp. 493-496, "An Angiogenic Factor Isolated from Tumours: A Potent Low-Molecular Weight Compound".
Watt, Sandra Louise, Diss. Abst. vol. 42(9), Mar. 1982, No. 3673B "Purification and Partial Characterization . . . Walker 256 Rat Carcinoma.
Merck Index 9th Edition, 1978, p. 103.
Schur et al. Biol. Abst., vol. 71(1), No. 4280, 1980, "Stimulation by a Low--Molecular Weight Angiogenic Factor . . . ".
Brown et al, Biol. Abst., vol. 70(3), No. 17559, 1980, "Angiogenic Factor from Synovial Fluid Resembling that from Tumors".
Fenselau et al., J. Biol. Chem. vol. 256, No. 18, Sep. 1981, pp. 9605-9611, "Tumor Angiogenic Factor".
R. A. Brown, et al, The Lancet, "Angiogenic Factor from Synovial Fluid Resembling that from Tumors", pp. 682-685, Mar. 29, 1980.
Chemical Abstracts, vol. 92, p. 469, "An Angiogenic Factor Isolated from Tumors: A Potent Low-Molecular-Weight Compound", Weiss, et al, 1980.
S. R. Ayad, et al, "DNA Uptake by a Mutant Streain of Lymphoma Cells", Nature, vol. 220, No. 5162, pp. 35-38, Oct. 5, 1968.
A. White, et al, "Changes in Camp and Fibronectin Concentrations in Adherent and Suspension Forms of a Hybrid Cell Line", Exp Cell Res 129, (1980), pp. 303-312.

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