Analytical utilization of phagocyte cell lines

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 29, 4351722, 43524026, 435941, 436 63, 436172, 436503, 436519, 436821, 935 90, 935103, 935110, C12N 500, C12Q 102, G01N 33567, G01N 33569

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047374551

ABSTRACT:
The property of phagocyte cells to show chemiluminescence when activated by certain chemical or immunological agents is used for a qualitative and quantitative analysis of such agents in biological fluids. A variety of factors such as opsonizing anti-bodies, complement, inhibitors, membrane-specific anti-bodies, and their antigens, membrane-specific lectins, lymphokines, endotoxins, toxic substances and others can be analyzed in this way. The new method finds application in the biotechnical, clinical, immunological and pharmacological fields, with a special preference for toxicological screening of pharmaceuticals. The phagocyte cells needed for the analysis can be derived from a continuous phagocyte cell line and a method of producing such a cell line is provided.

REFERENCES:
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E. Tzehoval et al., Journ. Immunology, 132, 1741-1747, 1984.
C. S. F. Easmon et al., Immunology 41, 67-74, 1980.
V. G. Hemming et al., Journ. Clin. Invest. 58, 1379-1387, 1976.
C. R. Johnson et al., Journ. Immunol. Meth. 67, 108-117, 1984.
P. Stahl et al., Journ. Cell Biol. 93, 49-56, 1982.
E. Tzehoval et al., In S. J. Norman et al. (Eds.) Macrophages and Natural Killer Cells, Plenum Press, New York, pp. 445-451, 1982.

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