Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-03-29
2005-03-29
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007220, C435S007910
Reexamination Certificate
active
06872539
ABSTRACT:
An analytical system for rapid detection and identification of different analytes directly from a test sample by mixing test material with a germinogenic source and enzyme-free spores, allowing the mixture to stand for a time to allow analyte-induced spore germination and subsequent de novo activity of an enzyme capable of producing a germinant in the presence of the germinogenic source and detecting the presence of a germination-derived product. The germinant which is formed promotes further spore germination with concomitant additional de novo enzyme synthesis or activation which results in a propagating cascade of analyte-independent germination after which a germination-derived product can be easily detected. The technique is particularly efficient to conduct thousands of parallel assays in an array of microscopic wells.
REFERENCES:
patent: 5614375 (1997-03-01), Citri
patent: 6228574 (2001-05-01), Rotman
patent: 6596496 (2003-07-01), Rotman
patent: WO 9521936 (1995-08-01), None
Hagerman et al., Analytical Biochemistry, vol. 151, pp. 334-342 (1985).
Housel James
Jacobs Bruce F.
Lucas Zachariah
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