Analytical reagent and method

Details Analytical reagent and method Analytical reagent and method

1977-08-02

1981-12-22

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 260112B, 424 12, G01N 3316, A61K 4300, C07G 700

Patent

active

043070710

ABSTRACT:
A reagent is disclosed for determining the concentration of a ligand in blood serum. The reagent includes a protenaceous, three-dimensional, serum-insoluble complex which is readily collected by centrifugation, the complex comprising non-specific immunoglobulins, anti-ligand immunoglobulins, immunoglobulins which are antibodies to the previously mentioned immunoglobulins and which are complexed therewith, and a labeled ligand complexed with the anti-ligand immunoglobulins. The reagent is particularly useful in competitive binding analyses such as radioimmunoassays.

REFERENCES:
patent: 2957808 (1960-10-01), Campbell
patent: 3911096 (1975-10-01), Chopra
patent: 3928553 (1975-12-01), Hollander
patent: 3951748 (1976-04-01), Devlin
patent: 3995019 (1976-11-01), Jerome
patent: 4018883 (1977-04-01), Parslow
patent: 4021534 (1977-05-01), Lafontaine
patent: 4034073 (1977-07-01), Weetall
patent: 4069352 (1978-01-01), Parsons, Jr.
Hales, et al., Biochem. J., 83, 1963, pp. 137-146.
Eckert, Angewandt Chemie, vol. 15, No. 9, Sep. 1976, pp. 525-533.
Siegel et al., J. Clin. Endocrinology Met., 37, 1973, pp. 526-532.

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