Analytical method using tetrazolium salt indicators having a ref

Details Analytical method using tetrazolium salt indicators having a ref Analytical method using tetrazolium salt indicators having a ref

1990-09-19

1992-06-30

Housel, James C.

Chemistry: analytical and immunological testing

Optical result

With reagent in absorbent or bibulous substrate

436172, 436164, 422 56, 422 57, 435 26, 435 25, 435805, G01N 2172

Patent

active

051262754

ABSTRACT:
General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.

REFERENCES:
patent: 4552848 (1985-11-01), Yudelson et al.
patent: 4940660 (1990-07-01), Hirai et al.
patent: 4978613 (1990-12-01), Bieniarz et al.
STN accession No. CA80(21):117518t Shannon et al; "Ultrastructural localization of monoamine oxidase (MAO) with tryptamine and a new tetrazolium salt, 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl) tetrazolium chloride (BSPT)" J. Histochem. Cytochem. 22(3) 170-82.
STN file accession No. CA77(17):110708j Kalina et al; "Nonosmiophilic tetrazolium salts that yield osmiophilic, lipophobic formazans for ultrastructural localization of dehydrogenase activity" J. Histochem. Cytochem. 20(9) 685-95.
STN file accession No. CA110(23):208931b.

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