Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Reexamination Certificate
2007-02-08
2009-10-13
Gakh, Yelena G (Department: 1797)
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
C436S161000, C435S007100, C435S023000, C210S634000, C210S136000, C210S656000, C073S061560, C422S069000, C422S105000
Reexamination Certificate
active
07601542
ABSTRACT:
Analytical methods using hydrogen/deuterium exchange are provided which reduce or eliminate the back-exchange of deuterium for hydrogen. The methods, which are useful in protein and peptide mapping, include the steps of (a) providing a peptide or protein comprising a solvent accessible hydrogen; (b) exchanging the solvent accessible hydrogen for a deuterium; (c) separating the peptide or protein with supercritical fluid chromatography; and (d) analyzing by mass spectrometry the mass of the separated peptide or protein. Supercritical fluid chromatography enables the observation of fast exchanging hydrogen atoms missed using conventional liquid chromatography methods.
REFERENCES:
Bolanos, Ben, et al., SFC/MS in drug discovery at Pfizer, La Jolla, 2004, International Journal of Mass Spectroetry, vol. 238, pp. 85-97.
Wang, et al., “Conformational and Dynamic Chanages of Yersinia Protein Tyrosine Phosphatase Induced by Ligand Binding and Active Site Mutation and Revealed by H/D Exchange and Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry,” Biochemistry, 37:15289-99 (1998).
Emmett, et al., “Supercritical Fluid Chromatography Reduction of Hydrogen/Deuterium Back Exchange in Solution-Phase Hydrogen/Deuterium Exchange with mass Spectrometric Anaylysis,” Anal. Chem. 78:7058-60 (2006).
Wikipedia, Title: “High Performance Liquid Chromatography,” Feb. 8, 2006, pp. 1-3.
Emmett Mark R.
Greig Michael J.
Kazazic Sasa
Marshall Alan G.
Florida State University Research Foundation
Gakh Yelena G
Sutherland & Asbill & Brennan LLP
Xu Robert
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