Analytical method for protein mapping using...

Details Analytical method for protein mapping using... Analytical method for protein mapping using...

2011-06-14

2011-06-14

Gakh, Yelena G (Department: 1777)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C436S161000, C435S007100, C435S023000, C210S634000, C210S136000, C210S656000, C073S061560, C422S069000

Reexamination Certificate

active

07960179

ABSTRACT:
Analytical methods using hydrogen/deuterium exchange are provided which reduce or eliminate the back-exchange of deuterium for hydrogen. The methods, which are useful in protein and peptide mapping, include the steps of (a) providing a peptide or protein comprising a solvent accessible hydrogen; (b) exchanging the solvent accessible hydrogen for a deuterium; (c) separating the peptide or protein with supercritical fluid chromatography; and (d) analyzing by mass spectrometry the mass of the separated peptide or protein. Supercritical fluid chromatography enables the observation of fast exchanging hydrogen atoms missed using conventional liquid chromatography methods.

REFERENCES:
Suchanova, Bohumila, et al. Foding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry, 2008, Microbial Cell Factories, vol. 7(12), pp. 1-20.
Chester, Thomas L., The Road to Unified Chromatography: The Importance of Phase Behavior Knowledge in Supercritical Fluid Chromatography and Related Techniques, and a Look at Unification, Microchemical J. 61, pp. 12-24 (1999).

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