Analytical method for detecting alkaline sphingomyelinase...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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Reexamination Certificate

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11359619

ABSTRACT:
An analytical fluorometric method and a kit for use in such method are disclosed for assessing the presence of alkaline sphingomyelinase (SMase) in the stool of a patient in need of such an assessment since alkaline SMase is a marker of serious pathological states, such as colon cancer.

REFERENCES:
patent: 6265179 (2001-07-01), Zhou et al.
patent: WO 03/056031 (2003-07-01), None
Hertervig et al, “Alkaline Sphingomyelinase Activity is Decreased in Human Colorectal Carcinoma,” (Cancer), 1997, vol. 79, pp. 448-453.
Duan et al, “Effects of Ursodeoxycholate and Other Bile Salts on Levels Of Rat Intestinal Alkaline Sphingomyelinase: A Potential Implication in Tumorigenesis,” (Dig Dis & Sci), Jan. 1998, vol. 43, No. 1, pp. 26-32.
Molecular Probes Inc., Invitrogen Corporation, “Product Information: Amplex Red Sphingomyelinase Assay Kit (A12220)”.
Denisova Natalia A et al: “Role of membrane lipids in regulation of vulnerability to oxidative stress in PC12 cells: Implication for aging. ” Free Radical Biology & Medicine, vol. 30, No. 6, Mar. 15, 2001, pp. 671-678, XP002255204 ISSN: 0891-5849 p. 673.
Sofic E et al: “Antioxidant and pro-oxidant capacity of catecholamines and related compounds. Effects of hydrogen peroxide on glutathione and spingomyelinase activity in pheochromocytoma PC12 cells: Potential relevance to age-related diseases.” Journal of Neural Transmission, vol. 108, No. 5, 2001, pp. 541-557, XP002255205 ISSN: 0300-9564 p. 544-p. 545.
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He Xingxuan et al: “A Fluorescence-Based, High-Throughput Sphingomyelin Assay for the Analysis of Niemann-Pick Disease and Other Disorders of Sphingomyelin Metabolism.” Analytical Biochemistry, vol. 306, No. 1, Jul. 1, 2002, pp. 115-123, XP002255206 Jul. 1, 2002, ISSN: 003-26797 Abstract.
Amplex® Red Sphingomyelinase Assay Kit (A12220), Molecular Probes, Product Information, Revised: Oct. 1, 2004.
Invitrogen Corporation Molecular Probes, “Product Information: Amplex Red Sphingomyelinase Assay Kit (A1220)”, 4 pages.
Biocompare Website Printout, “Amplex Red Sphingomyelinase Assay Kit”, first available on web 1999, 3 pages.
Email Communication Printout, Amplex Red Sphingomyelinase Assay Kit (A1222), Product Availability May 12, 1999, dated Oct. 20, 2005.
Molecular Probes Invitrogen Detection Technologies, “Amplex Red Sphingomyelinase Assay Kit (A12220)” Product Information, Revised Oct. 2004, p. 1-4.

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