Analytical method and apparatus for determining fluorescence or

Details Analytical method and apparatus for determining fluorescence or Analytical method and apparatus for determining fluorescence or

1986-04-21

1988-10-18

Marcus, Michael S.

Chemistry: analytical and immunological testing

Automated chemical analysis

With conveyance of sample along a test line in a container...

2504591, 422 64, 422 65, 422 67, 422 52, 436172, G01N 2164, G01N 3502

Patent

active

047787635

ABSTRACT:
A turntable holding a train of reaction containers thereon is continuously turned by one revolution plus an angle for a share of one container and then stopped. This makes one cycle. On the route of transfer of the reaction container, there are provided a light irradiation position and a light collection position.
The reaction container into which a sample and a marker reagent have been introduced is transferred such that it does not stop at the irradiation position and the light collection position. The reaction container to which a monochromatic light for excitation has been irradiated at the irradiation position is transferred toward the light collection position. Fluorescence coming from the light collection position is lead to a photodetector, and the concentration of the target substance in the sample is determined from the detected fluorescence after arithmetic processing.
Since the light collection position is provided at a place different from the irradiation position in the present apparatus, it is possible to measure fluorescence originating from the sample which is substantially free from the influence of background fluorescence.

REFERENCES:
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patent: 4313735 (1982-02-01), Yamashita et al.
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patent: 4407964 (1983-10-01), Elings et al.
patent: 4438329 (1984-03-01), Ford et al.
patent: 4485308 (1984-11-01), Rabatin

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