Analytical method and advice

Details Analytical method and advice Analytical method and advice

2005-06-07

2005-06-07

Nguyen, Bao-Thuy L. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S287900, C435S288300, C436S518000, C436S528000, C436S530000, C436S161000, C436S162000

Reexamination Certificate

active

06902889

ABSTRACT:
The invention relates to an analytical chromatographic method which comprises the steps of:a) providing a membrane type flow matrix attached to a liquid-impervious backing, which flow matrix permits a capillary force assisted lateral fluid flow therethrough, and at least a part of which flow matrix contains ion-exchange functions;b) treating the flow matrix to reduce or eliminate unspecific adsorption properties of the flow matrix;c) applying to the flow matrix a sample containing at least two components;d) initiating a first lateral flow of aqueous fluid to transport the sample through the flow matrix and separate said components therein;e) interrupting the lateral flow; and eitherf1) detecting at least one of the separated components on the flow matrix in the position reached by the respective component when the flow was interrupted; orf2a) initiating a second flow of aqueous fluid to transport the components in a direction substantially transverse to the direction of the first lateral flow;f2b) interrupting the second lateral flow; andf2c) detecting at least one of the separated components on the flow matrix in the position reached by respective component when the flow was stopped.The invention also relates to a chromatographic device comprising a membrane type flow matrix attached to a liquid-impervious backing, which membrane permits a capillary force assisted lateral fluid flow therethrough and at least a part of which is modified to support ion-exchange functions.

REFERENCES:
patent: 4313906 (1982-02-01), Filipi et al.
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Stedman's Medical Dictionary. 26thEdition.Williams & Wilkins. 1995. p. 336.

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