Analytical element for theophylline determination using buffer i

Details Analytical element for theophylline determination using buffer i Analytical element for theophylline determination using buffer i

1988-07-25

1991-07-02

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435184, 435805, 435810, 436825, 422 55, 422 56, 422 57, C12Q 142

Patent

active

050285289

ABSTRACT:
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises a porous spreading zone, a subbing zone and at least one other zone. The zones are in fluid contact with each other. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The subbing zone contains polyvinylpyrrolidone and a buffer for maintaining a pH of 9 or less.

REFERENCES:
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patent: 4782017 (1988-11-01), Frickey et al.
patent: 4900665 (1990-02-01), Terashima et al.
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Ansari et al., Clinica Chimica Acta, 118, pp. 135-139 (1982).
U.S. Ser. No. 692,473, Norton, filed Jan. 18, 1985.
U.S. Ser. No. 900,065, Frickey et al., filed Aug. 25, 1986.

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