Analytical, element, composition and method using modified apo-h

Details Analytical, element, composition and method using modified apo-h Analytical, element, composition and method using modified apo-h

1995-10-10

1998-07-07

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 422 57, 435 793, 435 794, 435 795, 435 28, 435962, 435968, G01N 3353, C12Q 128

Patent

active

057767149

ABSTRACT:
Modified apo-peroxidases have been found useful in analytical methods to remove the bias caused by the presence of hemoglobin in the test specimens. While apo-peroxidases are known to remove serum interferents, they fail to overcome the hemoglobin bias, particularly when dry coated analytical elements are used in the assays. Blocking the nitrogen atoms of the imidzolyl groups of the histidine amino acids of the apo-peroxidase prevents catalytic activity of the protein in the presence of hemoglobin.

REFERENCES:
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4376165 (1983-03-01), Hornby et al.
patent: 4598044 (1986-07-01), Kricka et al.
patent: 4828983 (1989-05-01), McClune
Miles, Methods in Enzymology, 47, pp. 431-442 (1977).
Gurd, Methods in Enzymology, 11, pp. 532-541 (1967).

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