Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1986-04-21
1989-03-14
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 25, 435 28, 435810, C12Q 134
Patent
active
048123994
ABSTRACT:
An analytical element can be used for the determination of either creatinine or creatine or both. The element contains creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance. The creatine amidinohydrolase is present in a manner such that it is substantially inert to the leuco dye. The creatinine amidohydrolase is present in a rate limiting amount. By measuring the amount of dye formation at particular times during the assay, either or both of the analytes can be determined with the same element.
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Mauck John C.
Mauck Linda A.
Norton Gary E.
Eastman Kodak Company
Rosen Sam
Tucker J. Lanny
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