Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1986-08-25
1988-11-01
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 7, 435184, 435805, 436822, C12Q 142, C12N 999
Patent
active
047820179
ABSTRACT:
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises a porous spreading zone and at least one other zone in fluid contact with the spreading zone. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The element also contains a buffer for maintaining the pH at 9 or less, substantially all of which is located in the spreading zone.
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Frickey Paul H.
Norton Gary E.
Eastman Kodak Company
Kepplinger Esther M.
Tucker J. Lanny
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