Analytical element and method for colorimetric determination of

Details Analytical element and method for colorimetric determination of Analytical element and method for colorimetric determination of

1984-09-26

1987-07-14

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

422 56, 435 19, 435 25, 435 28, 435805, C12Q 160, C12Q 128, C12Q 144

Patent

active

046802592

ABSTRACT:
An analytical element is designed for the colorimetric determination of total cholesterol in aqueous liquids, such as biological fluids. This element comprises an absorbent material and critical amounts of cholesterol ester hydrolase (from about 1500 to about 12,000 I.U./m.sup.2) and a nonionic surfactant (from about 5 and up to, but less than 11 g/m.sup.2). The use of these ranges of reagents reduces the potential for interference by triglycerides present in a test sample. Total cholesterol is determined with this element by detection of a color change resulting from a series of enzymatic reactions which produce hydrogen peroxide.

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Wiebe et al., Clin. Chem., 30(3), pp. 352-356 (1984).
Kodak Publication MP2-35, 1983 entitled "Kodak EKTACHEM Clinical Chemistry Products Test Methodology for Cholesterol Analysis."
Research Disclosure 201005, published Dec. 20, 1980 (Abstract).

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