Analytical element and method for alkaline phosphatase assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435805, C12Q 142

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active

045554846

ABSTRACT:
Disclosed herein is a dry analytical element and a method of using same for quantitatively detecting alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones which can be self-supporting or carried on a support. The first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitrophenyl phosphate, and the second reagent zone contains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.

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patent: 3905872 (1975-09-01), Forgione
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patent: 4132598 (1979-01-01), Modrovich
patent: 4306020 (1981-12-01), Meiattini
Bowers et al., Clin. Chem., 21, pp. 1988-1995, (1975).
McCombs et al., Clin. Chem., 18, pp. 97-104, (1972).

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