Analytical element and its use in a whole blood hemoglobin assay

Details Analytical element and its use in a whole blood hemoglobin assay Analytical element and its use in a whole blood hemoglobin assay

1984-06-05

1989-11-14

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

422 56, 422 57, 422 58, 435805, 436170, G01N 2178, G01N 3372

Patent

active

048807494

ABSTRACT:
Disclosed herein is an assay useful for the quantitative determination of hemoglobin in whole blood. This assay utilizes a multizone element comprising a registration zone comprising particulate barium sulfate distributed in a "blush" polymer, and a spreading zone having a void volume and an average pore size (e.g. greater than 5 microns) effective to accommodate whole blood. The element also contains an interactive composition capable of converting substantially all forms of hemoglobin into a detectable end product. One particularly useful interactive composition comprises a hemoglobin oxidizing agent (e.g. ferricyanide) and thiocyanate. Another useful interactive composition comprises an ionic surfactant, e.g. sodium octyl sulfate or sodium dodecyl sulfate.

REFERENCES:
patent: 3546131 (1970-12-01), Stern et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4057394 (1977-11-01), Genshaw
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4337222 (1982-06-01), Kitajima et al.
patent: 4340565 (1982-07-01), Kitajima et al.
patent: 4341257 (1982-07-01), Zander et al.
patent: 4430436 (1984-02-01), Koyama
Perutz et al., Biochem., 17, pp. 3640-3652, (1978).
Oshiro et al., Rinsho Byorei, 29(2), pp. 203-209, (1981).
Gersonde, J. Mol. Biol., 42, pp. 285-300, (1969).
Katz et al., Biochem., 12(4), pp. 710-713, (1973).
Gersonde, J. Mol. Biol., 14, pp. 37-47, (1965).

Affiliated with

Also associated with

Rating

Analytical element and its use in a whole blood hemoglobin assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Analytical element and its use in a whole blood hemoglobin assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Analytical element and its use in a whole blood hemoglobin assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1852748