Analytical dipstick for improved mixing and reduced reagent volu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 4, 435 5, 435 71, 435 75, 435 792, 435970, 435973, 435975, 436518, 436524, 436525, 436528, 436530, 436531, 436808, 436809, 436810, 422 58, C12Q 168

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055784461

ABSTRACT:
The analytical dipstick of the present invention facilitates analyte testing with reduced liquid reagent volumes and provides improved mixing. The dipstick includes an elongated member having a fluid displacing member at one end thereof and a ligand attached to a portion of the elongated member in a region adjacent to the displacing member. The displacing member has a configuration that substantially conforms with the inner side wall of an analytical well in which the dipstick is placed to displace fluid therein. When the displacing member is substantially centered at the bottom of the well, the portion of the dipstick supporting the ligand preferably is spaced from the inner side wall by a distance of at least about twice that between the displacing member and the inner side wall, measured along a line passing through the greatest lateral dimension of the displacing member. With this configuration, essentially all of the liquid at the bottom of the well is displaced upwardly so that the ligand is immersed therein. The relatively large space between the intermediate portion and the side wall of the well facilitates turbulent flow to enhance mixing in the region of the ligand.

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patent: 5126276 (1992-06-01), Fish et al.

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