Analytical determination of lipase

Details Analytical determination of lipase Analytical determination of lipase

1980-11-24

1982-08-31

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, C12Q 126, C12Q 144

Patent

active

043473130

ABSTRACT:
A test for lipase activity is provided wherein trilinolein, or an equivalent as defined herein, is employed in an emulsified form as a substrate. Reduced incubation times and improved accuracy are obtained by providing an excess of lipoxygenase to convert the linoleic acid product of lipolysis to linoleic acid hydroperoxide which is measurable with great accuracy at low concentrations by spectrophotometric or kinetic assay techniques, for example.

REFERENCES:
patent: Re29498 (1977-12-01), Meiattini
patent: 3689364 (1972-09-01), Hartel et al.
patent: 3838011 (1974-09-01), Hagen et al.
patent: 3917515 (1975-11-01), Goldberg
Whitaker, Principles of Enzymology for the Food Sciences, Marcel Dekker, Inc., New York, 1972, pp. 609, 610, 615.
Proelss et al., Clin. Chem., 23(3), 522-531 (1977).

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