Analytical composition, element and method for the determination

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

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422 56, 435 25, 435 28, 435805, 436904, C12Q 126, C12Q 128, C12Q 154

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047374578

ABSTRACT:
An analytical method for the determination of hydrogen peroxide utilizes a hydrazide or a hydrazine substituted with one or more electron withdrawing groups. The hydrazides or hydrazines react with phenolic color couplers in the presence of hydrogen peroxide and a peroxidative compound to form a dye detectable at a wavelength of 600 nm or greater. These materials can also be used to assay analytes, such as glucose and cholesterol, which can react to produce hydrogen peroxide.

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Gochman et al, Clin. Chem., 17(12), pp. 1154-1159 (1971), Automated Determination of Uric Acid with Use of a Uricase-Peroxidase System.
Gochman et al, Clin. Chem., 18(9), pp. 943-950 (1972); Application of a New Peroxide Indicator Reaction to the Specific, Automated Determination of Glucose with Glucose Oxidase.

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