Analyte detection using an active assay

Details Analyte detection using an active assay Analyte detection using an active assay

2006-04-05

2010-11-02

Gabel, Gailene R (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

C435S002000, C435S007100, C435S285200, C435S286100, C435S286200, C435S286500, C435S287200, C436S517000, C436S518000, C436S524000, C436S528000, C436S534000, C436S538000, C436S165000, C436S177000, C436S178000

Reexamination Certificate

active

07824927

ABSTRACT:
Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.

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