Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-04-02
1998-12-22
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 435174, 435177, 435181, 435964, 436518, 436528, 436532, 436537, 436544, 436545, 436819, 530810, 530812, 530816, C12N 1100, C12N 1106, G01N 33547, G01N 33532
Patent
active
058517782
ABSTRACT:
A trifunctional conjugate is provided having three chemical moieties attached through a spacer moeity. At least two of the chemical moieties are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected to impart certain steric properties to the conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the chemical moieties sterically inhibits the binding of a different macromolecule to another chemical moiety. In another embodiment, the binding of a first chemical moiety to a macromolecule restricts the subsequent binding of a second chemical moiety to a proximate location on the same macromolecule. The three chemical moieties are preferably a nitrophenylazido residue, a phenyl boronic acid residue, and a solid support or a label such as biotin. The spacer is preferably cysteine, lysine, glutamic acid, pyroglutamic acid, S-acetylmercaptosuccinic anhydride or .omega.-carbobenzoxylysine. The conjugate is useful in immunoassays and for targeted labeling of proteins. In an assay, a sample is contacted with the conjugate, a limited quantity of analyte binding partner and an excess of small molecule binding partner. Presence of the analyte is determined by detecting the amount of analyte binding partner diverted away from analyte attached to the spacer of the conjugate.
REFERENCES:
patent: 5661019 (1997-08-01), Oh et al.
Oh Chan S.
Sternberg James C.
Beckman Instruments Inc.
Kivinski Margaret A.
May William H.
Naff David M.
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