Analysis of samples utilzing capillary electrophoresis

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2726, B01D 5702

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active

051204134

ABSTRACT:
Method and electrolyte buffer useful in the analysis of samples comprising glycoproteins by capillary zone electrophoresis. The buffer comprises at least one agent capable of complexing with sugar moieties of glycoproteins. The buffer preferably further comprises at least one pH modifier. An embodiment of the buffer comprises boric acid as the complexing agent and sodium hydroxide as the pH modifier.

REFERENCES:
Fujiwara, S. & Honda, S.; "Determination of Cinnamic Acid and Its Analogues by Electrophoresis in a Fused Silica Capillary Tube"; Anal. Chem. 58: 1811-1814 (1986).
Otsuka, K. et al; "Quantitation and Reproducibility in Electrokinetic Chromatography with Micellar Solutions"; J. Chrom. 396 350-354 (1987).
Chen, Fu-Tai A. et al; "Capillary Electrophoresis-A New Clinical Tool"; Clin. Chem. 77/1:14-19 (1991).
Gordan, M. J. et al; "Protocol for Resolving Protein Mixtures in Capillary Zone Electrophoresis"; Anal. Chem. 63:69-72.
Jorgenson, J. W. and Lukacs; K. D.; "Capillary Zone Electrophoresis"; Science 222:226-272 (1983).
Lauer, H. H. & McManigill, D.; "Capillary Zone Electrophoresis of Proteins in Untreated Fused Silica Tubing"; Anal. Chem. 58:166-170 (1986).
Susumu Honda et al "Simultaneous Determination of Reducing Monosaccharides by Capillary Zone Electrophoresis as the Borate Complexes of N-2-Pyridylglycamines" Analytical Biochemistry 176 (1989) 72-77.
Susumu Honda et al "Analysis of the Oligosaccharides in Oval-bumin by High-Performance Capillary Electrophoresis", Analytical Biochemistry 191, (1990), 228-234.
Sabrina Hoffstetter-Kuhn et al "Influence of Borate Compexation on the Electrophoretic Behavior of Carbohydrates in Capillary Electrophoresis" Analytical Chemistry 63 (1991) 1541-1547.
Henk H. Lauer & Douglass McManigill "Capillary Zone Electrophoresis of Proteins in Untreated Fused Silica Tubing" Analytical Chemistry, 58 (1986) 166-170.
Manuel J. Gordon et al "Protocol for Resolving Protein Mixtures in Capillary Electrophoresis" Analytical Chemistry 63 (1991) 69-72.

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