Analysis of samples by electrophoresis using a charge coupled de

Details Analysis of samples by electrophoresis using a charge coupled de Analysis of samples by electrophoresis using a charge coupled de

1988-06-06

1989-10-17

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726

Patent

active

048744921

ABSTRACT:
Light from the pattern of spots of bands resulting from electrophoresis is detected by a two-dimensional charge coupled device, visualization of the spots or band being achieved by irradiating the gel with a U/V source to stimulate fluorescent markers preferably applied before running of the gel. The charge coupled device is cooled to suppress thermal dark current and, more particularly, to improve sensitivity and dynamic range.

REFERENCES:
patent: 4666578 (1987-05-01), Yamamoto
Toda et al, "Microcomputer-Aided Two-Dimensional Densitometry", Electrophoresis '83, (1984), pp. 139-146.
Smith et al, "Fluorescence Detection in Automated DNA Sequence Analysis", Nature, vol. 321 (Jun. 12, 1986), pp. 674-679.
Bonazzola et al, "Charged Coupled Devices as Autoradiography Imagers", IEEE Transactions on Nuclear Science, vol. NS-32, No. 1 (Feb. 1985), pp. 567-570.
Sutherland et al, "Electronic Imaging System for Direct and Rapid Quantitation of Fluorescence from Electrophoretic Gels", Analytical Biochemistry 163 (1987), pp. 446-457.

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