Analysis of samples by capillary electrophoretic immunosubtracti

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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C25B 700

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active

052289605

ABSTRACT:
Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ("CEI") of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises the steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

REFERENCES:
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White, W. A. & Attwood, E. C. "Immunofixation and Immunosubtraction on Agarose Gel: An Acid in Typing of Monoclonal Gammopathies." Biochem. Clin. 10:571-574 (1986).
Merlini, G. et al., "Identification of Specific Plasma Proteins Determining the Agarose Cel Electrophoresis by the Immunosubtraction Technique." J. Clin. Chem. Biochem. 21:841-844 (1983).
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Sun, T. et al. "Study of Gammopathies with Immunofixation Electrophoresis." AJCP 72/1: 5-11 (1979).
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