Analysis of polymeric protein and protein products

Details Analysis of polymeric protein and protein products Analysis of polymeric protein and protein products

1985-11-26

1987-10-20

Bashore, S. Leon

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 15, 436175, 435 23, 435 24, 250282, G01N 3368

Patent

active

047014199

ABSTRACT:
Methods are provided for determining the presence or absence of ragged ends in polymeric proteins or protein products, by means of fast atom bombardment and high mass mass spectrometry (preferably high field magnet mass spectrometry). The methods may be modified for locating N- or C-terminal peptide in a polymeric protein or protein product and for assigning disulphide bridges in polymeric protein or protein products. The methods are applicable to other biopolymers such as nucleotides and carbohydrates.

REFERENCES:
patent: 3959307 (1976-05-01), Wittmann
patent: 4065412 (1977-12-01), Dreyer
patent: 4224031 (1980-09-01), Mee et al.
patent: 4554254 (1985-11-01), Krystal
Miller, M. J., Loudon, G. M., J Am Chem Soc (U.S.A.), vol. 97, No. 18, U.S.A. 23-230M, Sep. 3, 1975.

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