Analysis of lactic acid or lactate using lactate oxidase

Details Analysis of lactic acid or lactate using lactate oxidase Analysis of lactic acid or lactate using lactate oxidase

1976-12-10

1979-09-04

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435189, 435805, 435885, C07G 702, G01N 3114

Patent

active

041667638

ABSTRACT:
Compositons and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.

REFERENCES:
patent: 3907642 (1975-09-01), Richmond
patent: 3992158 (1976-11-01), Przybylowicz et al.
Barman, Enzyme Handbook, vol. 1, Spring-Verlag New York, Inc. 1969, p. 111.
Eichel et al., J. Biol. Chem., vol. 237, No. 3, 1962, pp. 940-945.
London, J. Bact., vol. 95, No. 4, 1968, pp. 1380-1387.
Sutton, J. Biol. Chem., vol. 226, 1957, pp. 395-405.
Dixon et al., Enzyme, Academic Press Inc., Publishers, New York (1964), pp. 680 & 681.

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