Analysis of lactic acid or lactate using lactate oxidase

Details Analysis of lactic acid or lactate using lactate oxidase Analysis of lactic acid or lactate using lactate oxidase

1981-08-31

1985-10-29

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435189, 435805, 435885, C12Q 126, C12Q 128, C12N 902, C12R 146

Patent

active

RE0320161

ABSTRACT:
Compositions and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.

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Eichel et al., Respiratory Enzyme Studies in Tetrahymena pyriformis: The Journal of Biological Chemistry, vol. 237, No. 3, 1962, (pp. 940-945).
London J., Regulation and Function of Lactate Oxidation in Streptococcus faecium, Journal of Bacteriology, vol. 95, No. 4, 1968, (pp. 1380-1387).
Sutton, W. B., Mechanism of Action and Crystallization of Lactic Oxidative Decarboxylase from Mycobacterium phlei, J. Biol. Chem., vol. 226, 1957, (pp. 395-405).
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