Analysis of isoenzyme patterns

Details Analysis of isoenzyme patterns Analysis of isoenzyme patterns

1973-11-27

1977-03-15

Naff, David M.

Textiles: fiber preparation

195 63, 424 2, 424 12, C12K 104, G01N 3114

Patent

active

040122859

ABSTRACT:
Isoenzyme patterns of an enzyme in biological fluids, extracts, and/or excretions are quantitatively determined by precipitating at least 90% of an isoenzyme antigen with a corresponding heterologous antiserum and comparing the remaining enzyme activity with the total activity with the use of a conventional agent for enzyme activity determination.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3932221 (1976-01-01), Pfleiderer
Markert et al., Immunochemical Properties of Lactate Dehydrogenase Isoenzymes, Annals New York Academy of Sciences, 103, 1963 (pp. 915-929).
Van Loon et al., "Photometric Method for Blood Amylase by Use of Starch-Iodine Color," Am. J. Clin. Pathol. 22:1134 (1952).
McGeachin et al., "Serological Differentiation of Amylase Isozymes," Ann. N.Y. Acad. Sci., 94, pp. 996-1003 (1961).
Assicot et al., "Production of Antibodies to Catechol-O-Methyl-Transferase (EC2.1.1.6) of Rat Liver," Biochem. Pharmacology 18, pp. 1893-1898, (1969).
J.S. Nisselbaum et al., "Reactions of Lactic Dehydrogenase From Various Rabbit Organs With Antirabbit Muscle Lactic Dehydrogenase," J. of Biol. Chem., vol. 234 (12) pp. 3276-3280 (1959).
McGeachin et al., "Serological Differentiation of Amylase Isozymes," Ann. N.Y. Acad. Sci., 94, pp. 996-1003 (1961).

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