Analysis of gene expression, methods and reagents therefor

Details Analysis of gene expression, methods and reagents therefor Analysis of gene expression, methods and reagents therefor

1998-01-08

1999-11-09

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

059811900

ABSTRACT:
The present invention is directed to methods and reagents for performing rapid, detailed analysis of the spatial and temporal expression of genes. The method uses linker tags in a head to tail array in a serial analysis of gene expression.

REFERENCES:
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patent: 5695937 (1997-12-01), Kinzler et al.
Kikuya Kato, (1995) Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes, Nucleic Acids Research, (18): 3685-3690.
Marc Lemaitre, et al., (1987) Specific antiviral activity of a poly (l-lysine)-conjugated oligodeoxyribonucleotide sequence complementary to vesicular stomatitis virus N protein mRNA initiation site, Proc. Natl. Acad. Sci. USA, 84: 648-652.
Paul Unrau and Kenneth V. Deugau, Non-cloning amplification of specifi DNA fragments from whole genomic DNA digests using DNA `indexers`, AECL Research, Chalk River Laboratories, Chalk River, Ontario K0J 1JO, Canada.
Victor E. Velculescu, et al., (1995) Serial Analysis of Gene Expression, Science 270:484-487.
RNA Polymerase From E. coli is a Multisubunit Enzyme, Part V, Genetic Information, RNA Synthesis and Splicing.
Zaug et al, "The tetrahymena ribozyme acts like an RNA restriction endonuclease", Nature 324:429-433, Dec. 1986.
New England Biolabs Catalog, p. 13, Jan. 1996.

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