Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1993-07-09
1995-12-05
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041828, 204299R, 536 187, 536 55, G01N 2726, G01N 27447
Patent
active
054725828
ABSTRACT:
The present invention provides for the use of the fluorescent label 2-aminoacridone for use in separating carbohydrate mixtures and analyzing the structure of carbohydrates. Carbohydrates for analysis may be labeled by 2-aminoacridone and subsequently separated from one another by electrophoresis. The electrophoresis may be in one or two dimensions. Band produced by the electrophoresis may be visualized and quantitated directly by UV illumination or by means of a charge coupled device for photoelectric detection. 2-aminoacridone labelling of carbohydrate may also be used to analyze the structure of carbohydrates by cleaving (or adding to) various 2-aminoacridone labeled carbohydrates by carbohydrate modifying enzymes of known specificity, and subsequently separating the carbohydrates by electrophoresis in either one or two dimensions. The subject invention also provides for kits for performing 2-aminoacridone labelling and electrophoresis.
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Astromed Limited
Halluin Albert P.
Niebling John
Starsiak Jr. John S.
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