Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1995-05-30
1999-01-12
Tsang, Cecilia J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 25, 435 26, 435 39, 435190, 436 12, 436 63, 436516, 204450, 204451, 204452, C12Q 100, G01N 3348
Patent
active
058586440
ABSTRACT:
A method for detecting an analyte in a sample uses both the specificity of an enzymatic reaction and the separation power of capillary electrophoresis. In general, the method comprises: (1) subjecting a first aliquot of the sample to an analytical technique such as capillary electrophoresis, which generates a first output such as an electropherogram; (2) reacting a second aliquot of the sample in an enzyme-catalyzed reaction converting the analyte into a product, the product being detectable by the analytical technique; (3) subjecting the second aliquot to the analytical technique to generate a second output; (4) in the case of electrophoresis, measuring the absorbance of the first and second outputs (electropherograms) as a function of migration distance along the electropherogram at at least one wavelength at which either the analyte or the product absorbs to produce a first absorbance scan and a second absorbance scan; and (5) comparing the first absorbance scan with the second absorbance scan to detect the analyte. The reaction can involve a coenzyme and the analytical technique can be directed to the coenzyme. Alternatively, at least two enzymes can be used, the first enzyme generating a first product that is then acted upon by the second enzyme.
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Beckman Instruments Inc.
Farah David A.
Hampson Gary T.
May William H.
Mohamed Abdel A.
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