Analysis of a sample to determine its characteristic cycle time

Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence

Reexamination Certificate

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C250S459100, C356S317000, C422S082070

Reexamination Certificate

active

07098039

ABSTRACT:
An analysis for determining a characteristic cycle time of a sample. Active elements in the sample are excited with sufficient intensity and duration larger than the characteristic cycle time that at least some of the active elements are re-excited to an excited state substantially immediately following relaxation to a ground state, detecting quanta emitted by the active elements in the sample to obtain a detected signal, and analyzing the detected signal to derive the characteristic cycle time. The number of active elements in the sample and the intensity of the excitation are such that quanta are detected in a stream in which individual quanta are distinguishable from each other.

REFERENCES:
patent: 4421860 (1983-12-01), Elings et al.
patent: 5254477 (1993-10-01), Walt
patent: 5631169 (1997-05-01), Lakowicz et al.
patent: WO94/18218 (1994-08-01), None
patent: WO96/27798 (1996-09-01), None
Soper et al., Detection and identification of single molecules in solution, 1992, J. Opt. Soc. Am. B, vol. 9, No. 10, pp. 1761-1769.
Patent Abstracts of Japan vol. 011, No. 137 (P-572), (May 1987) & JP61 277060 A (Joko: KK), (Dec. 1986).

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