Analysis element and method for determination of an analyte in a

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

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422 56, 422 57, 422 58, 435 792, 435 793, 435 794, 435805, 435810, 435967, 435970, 435973, 436164, 436169, 436514, 436530, 436805, 436807, 436810, G01N 33543, G01N 33558

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active

054242207

ABSTRACT:
The invention addresses an analysis element for the determination of an analyte according to the principle of a heterogeneous immunoassay. Said analysis element is made of a chromatographic porous carrier material and has a reaction zone, at least two detection zones and absorptive zones following said detection zones. The reaction zone contains analyte-specific and labelled binding partners which are present in a number of soluble compartments which are close together, but nevertheless spatially separated. The detection zones are adjacent to the reaction zones and contain an immobilized binding reagent for one of the binding partners present in the reaction zone. At least one detection zone contains a binding reagent for the analyte-specific unlabelled binding partner.

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