Analysis based on flow restriction

Details Analysis based on flow restriction Analysis based on flow restriction

1995-04-24

1996-01-23

Warden, Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

422 58, 422 61, 422 681, 422 73, 435 72, 435 721, 4352872, 4352887, 4352871, 436164, 436524, 436809, G01N 2100

Patent

active

054863350

ABSTRACT:
Disclosed are devices and methods for detecting the presence of a preselected analyte in a fluid sample. The invention provides a device comprising a solid substrate, typically on the order of a few millimeters thick and approximately a 0.2 to 2.0 centimeters square, microfabricated to define a sample inlet port and a mesoscale flow system. A sample is passed through the mesoscale flow system, and the restriction or blockage of flow through the flow system is detected as a positive indication of the presence of the analyte. The mesoscale flow system includes in one embodiment a primary sample flow channel extending from the inlet port, and a fractal region, in fluid communication with the flow channel, comprising bifurcations leading to plural secondary flow channels. The device may be adapted for operation in conjunction with a pump, for example, to induce flow of a sample through the flow system. A detector may also be provided for detecting analyte induced changes in flow properties in the mesoscale flow system. The devices of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, such as viruses.

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