Analying polynucleotide sequences

Details Analying polynucleotide sequences Analying polynucleotide sequences

1997-09-09

2000-04-25

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

422 50, 422 681, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 253, C12Q 168

Patent

active

060542705

ABSTRACT:
This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.

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