Analogues of reporter groups as background reducers in hybridiza

Details Analogues of reporter groups as background reducers in hybridiza Analogues of reporter groups as background reducers in hybridiza

1994-01-14

1996-03-26

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 4, 435 71, 436501, 436164, 935 76, 935 77, 935 78, 935 81, C13Q 168, G01N 3353

Patent

active

055019528

ABSTRACT:
Assays for target molecules in and from cells and viruses, e.g. nucleic acids, wherein non-specific background is decreased by including an analogue of the reporter group, e.g. a non-fluorescent analogue such as fuchsin, of a fluorescent group such as fluorescein, to decrease non-specific backround.

REFERENCES:
patent: 4508821 (1985-04-01), Mansour et al.
patent: 4886741 (1989-12-01), Schwartz
patent: 4888278 (1989-12-01), Singer et al.
patent: 4996143 (1991-02-01), Heller et al.
patent: 5008186 (1991-04-01), Graystone et al.
patent: 5015568 (1991-05-01), Tsujimoto et al.
patent: 5028524 (1991-07-01), Fujisawa et al.
patent: 5032381 (1991-07-01), Bronstein
patent: 5086169 (1992-02-01), Mascarenhas
patent: 5202269 (1993-04-01), Ito et al.
patent: 5225326 (1993-07-01), Bresser et al.
patent: 5230998 (1993-07-01), Neurath et al.
Haase, A. T. Analysis of Viral Infections by In Situ Hybridization. In, In Situ Hybridization, Applications to Neurobiology. Eds., Valentino, Eberwine and Barchas, Oxford University Press, New York, 1987.
Ely, P., et al. A New In Situ Hybridization Technique for Spliced RNA Species Documents the Bone Marrow Origin of Pulmonary Macrophages in Chronic Myelogenous Leukemia. Blood, vol. 80, pp. 2321-2325 (1992).
Ledbetter, D. H., The `colorizing` of cytogenetics: is it ready for prime time? Human Molecular Genetics. vol. 1 pp. 297-299 (1992).
van den Berg, H., et al. Detection of Y Chromosome by In Situ Hybridization in Combination with Membrane Antigens by Two-Color Immunofluorescene. Laboratory Investigation, vol. 64, pp. 623-628 (1991).
Amman, R. I., et al. Fluorescent-Oligonucleotide Probing of Whole Cells for Determinative, Phylogenetic, and Environmental Studies in Microbiology. Journal of Bacteriology, vol. 172, pp. 762-770 (1990).
Landry, M. L., Nucleic Acid Hybridization in Viral Diagnosis. Clinical Biochemistry, vol. 23, pp. 267-277 (1990).
Schols, D., et al. Dextran Sulfate and Other Polyanionic Anti-HIV Compounds Specifically Interact with the Viral gp120 Glycoprotein Expressed by T-Cells Persistently Infected with HIV-1. Virology, Vol pp. 556-561 (1990).
Skidmore, A. F., et al. Characterization and use of the potent ribonuclease inhibitor aurintricarboxylic acid for the isolation of RNA from animal tissues. Biochem Journal vol. 263, pp. 73-80 (1989).
Turtinen, L. W., et al. Increased monokines in cytomegalovirus infected myelomonocytic cell cultures. Microbial Pathogenesis, vol. 7 135-145 (1989).
Holben, W. E., et al. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community. Applied and Environmental Microbiology, vol. 54, pp. 703-711 (1988).
Nederlof, P. M., et al. Three-Color Fluorescence In Situ Hybridization for the Simultaneous Detection of Multiple Nucleic Acid Sequences. Cytometry, vol. 10, pp. 20-27 (1988).
Miyada, C. G. and Wallace R. B., Oligonucleotide Hybridization Technique. Methods in Enzymology, vol. 154, pp. 94-107 (1987).
Contag, C. H., et al. Correlation between presence of lactate dehydrogenase-elevating virus RNA and antigens in motor neurons and paralysis in infected C58 mice. Virus Research, vol. 6, pp. 195-209 (1986).
Singer, R. H., et al. Optimization of in situ Hybridization Using Isotopic and Non-Isotopic Detection Methods. BioTechniques, vol. 4, pp. 230-250 (1986).
Weitgrefe, et al. Cloning of a gene Whose Expression in Increased in Scrapie and in Senile Plaques in Human Brain. Science, vol. 230, pp. 1177-1179 (1985).
Haase, A. T., et al. Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis. Proceedings of the National Academy of Sciences, vol. 82, pp. 3020-3024 (1985).
Korenberg (1992) Trends in BioTechnology vol. 10: 27-32.

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