Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1988-10-06
1993-07-20
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530395, 435691, 435 695, 435 699, 930145, C07K 1300
Patent
active
052294960
ABSTRACT:
Amplified expression of recombinant DNA products is achieved in hosts expressing protease that cleave at multi-basic amino acid residues. To this end, wild-type genes encoding the desired protein products are mutated by substituting codons or eliminating codons encoding multi-basic amino acid residues while maintaining the activity of the expressed protein product. Mutation of the desired gene can be conveniently carried out by site-specific in vitro mutagenisis.
REFERENCES:
patent: 4438032 (1984-03-01), Golde et al.
patent: 4675285 (1987-06-01), Clark et al.
Leatherbarrow et al., Protein Engineering 1(1) 1986, pp. 7-16.
Wong et al., Science 1985, pp. 810-815.
Miyatake et al., EMBO 4, 1985, p. 2561-2568.
Sparron et al., PNAS 82, 1985, pp. 292-296.
Gasson et al., Science 226, 1984, pp. 1339-1342.
Abboud et al., Blood 58, 1981, pp. 1150-1154.
Lusis et al., Blood 57, 1981, pp. 13-21.
Lusis et al., Nature 298, 1982, pp. 75-77.
Gough et al., Nature 309, 1984, pp. 763-767.
Lee et al., PNAS 82, 1985, pp. 4360-4364.
Stanley et al., EMBO 4, 1985 pp. 2569-2573.
Beggs, "Transformation of Yeast By a Replicating Hybrid Plasmid," Nature (London), 275:104-108 (1978).
Shortle et al., "Gap Misrepair Mutagenesis: Efficient Site-Directed Induction of Transition, Transversion, and Frameshift Mutations In Vitro,"Proc. Nat. Acad. Sci. (USA) 79:1588-1592 (1982).
Dalbadie-McFarland et al., "Oligonucleotide-Directed Mutagenesis as a General and Powerful Method for Studies of Protein Function," Proc. Nat. Acad. Sci. (USA) 79:6409-6413 (1982).
Kurjan and Herskowitz, "Structure of a Yeast Pheromone Gene (MF .alpha.), a Putative .alpha.-Factor Precursor Contains Four Tandem Copies of Mature .alpha.-Factor," Cell 30:933-943 (1982).
Craik, "Use of Oligonucleotides for Site-Specific Mutagenesis," BioTechniques, Jan. 1985, pp. 12-19.
Julius et al., Cell 37:1075-1089 (1984).
Achstetter et al., Embo, 173-7 (1985).
Taniyama et al., Biochem. Biophys. Res. Commun. 152:962 (1988).
Moonen et al, "Increased Biological Activity of Deglycosylated Recombinant Human Granulocyte/Macrophage Colony-Stimulating Factor Produced by Yeast or Animal Cells," PNAS 84:4428-31 (1987).
Shaw and Kamen, Cell 46:659-67 (1986).
Muellner and Kuehn, Cell 53:815-25 (1988).
Ernst et al., "O-Glycosylation and Novel Processing Events During Secretion of Alpha-Factor/GM-CSF Fusions by Saccharomyces Cerevisiae," Bio/Technology 5:831-34 (1987).
Brake et al., ".alpha.-Factor-Directed Synthesis and Secretion of Mature Foreign Proteins in Saccharomyces Cerevisiae," Proc. Natl. Acad. Sci. USA, vol. 81, pp. 4642-4646, 1984.
Bitter et al., "Secretion of Foreign Proteins from Saccharomyces Cerevisiae Directed by .alpha.-Factor Gene Fusions," Proc. Natl. Acad. Sci. USA, vol. 81, pp. 5330-6334, 1984.
Jones, "The Synthesis and Function of Proteases in Saccharomyces: Genetic Approaches," Ann. Rev. Genet., 18:233-70, 1984.
Deeley Michael C.
Gimpel Steven D.
Price Virginia L.
Draper Garnette D.
Immunex Corporation
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