Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1993-05-27
1995-02-28
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530395, 435 691, 435 695, 435 699, 930145, C07K 1300
Patent
active
053938704
ABSTRACT:
Amplified expression of recombinant DNA products is achieved in hosts expressing proteases that cleave at multi-basic amino acid residues. To this end, cDNAs encoding granulocyte-macrophage colony stimulating factor (GM-CSF) are mutated such that one or both of the arginine residues at positions 23 and 24 of the protein product are deleted or replaced by non-basic amino acid residues. The GM-CSF analogs thus obtained maintain the activity of the wild-type protein.
REFERENCES:
Beggs, "Transformation of yeast by a replicating hybrid plasmid", Nature, 275:104-08 (1978).
Moonen et al., "Increased biological activity of deglycosylated rh GM-CSF by yeast or animal cells", PNAS, 84:4428-31 (1987).
Bitter et al., "Secretion of foreign proteins from S. cerevisiae directed by .alpha.-factor gene fusions", PNAS, 81:5330-34 (1984).
Jones, "The Synthesis and Function of proteases in Saccharomyces", Ann. Rev. Genet., 18:233-70 (1984).
Deeley Michael C.
Price Virginia L.
Draper Garnette D.
Fitzgerald David L.
Immunex Corporation
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